early passage normal human female colon fibroblast ccd 18co cell line  (ATCC)

Journal: Cancer medicine

Article Title: Cancer-associated fibroblasts secrete Wnt2 to promote cancer progression in colorectal cancer.

doi: 10.1002/cam4.2523

Figure Lengend Snippet: FIGURE 1 Fibroblasts isolated from human colorectal cancer tissues. Isolated cells showed a spindle‐like shape, which makes no morphological distinction between CAFs and NFs (A). They were negative for the epithelial marker E‐cadherin (B). The expression of αSMA, vimentin, and FAP in these cells did not show a specific pattern between CAFs and NFs (C‐E)

Article Snippet: Human colon carcinoma cell lines (HCT116, DLD‐1, and HT‐29) and human colon normal fibroblast cell line (CCD‐18Co) were obtained from the American Type Culture Collection.

Techniques: Isolation, Marker, Expressing

Journal: Cancer medicine

Article Title: Cancer-associated fibroblasts secrete Wnt2 to promote cancer progression in colorectal cancer.

doi: 10.1002/cam4.2523

Figure Lengend Snippet: FIGURE 2 Analysis of gene expression profiling. The heat map demonstrated differentially expressed genes identified with RNA sequencing, including 283 upregulated genes and 301 downregulated genes in CAFs (A). Pathway analysis using Gene Set Enrichment Analysis revealed that the gene sets related to the Wnt signaling pathway were highly enriched in CAFs (B). CAFs had much higher WNT2 expression than NFs among the primary fibroblasts from each of the five cases (C). FPKM: fragments per kilobase of transcript per million mapped reads

Article Snippet: Human colon carcinoma cell lines (HCT116, DLD‐1, and HT‐29) and human colon normal fibroblast cell line (CCD‐18Co) were obtained from the American Type Culture Collection.

Techniques: Gene Expression, RNA Sequencing, Expressing